Например, в словаре.

Код AS3:
private const _tweens:Dictionary = new Dictionary();
// или в массиве
//private const _tweens:Array = new Array();
private function nextTween():void {
const tween:Tween = new Tween();
this._tweens[tween] = true;
//this._tweens.push(tween);
tween.addEventListener(TweenEvent.MOTION_FINISH, this.handler_tweenComplete, false, int.MAX_VALUE);
tween.start();
}
private function handler_tweenComplete(event:Event):void {
const tween:Tween = event.target as Tween;
delete this._tweens[tween];
//this._tweens.splice(this._tweens.indexOf(tween), 1);
}